Digital Quantification of the Enzyme-Linked Immunospot (ELISPOT)
نویسندگان
چکیده
منابع مشابه
Enzyme-linked immunospot assay (ELISPOT): Quantification of Th-1 cellular immune responses against microbial antigens.
Adaptive immunity is an important component to clearance of intracellular pathogens. The ability to detect and quantify these responses in humans is an important diagnostic tool. The enzyme-linked immunospot assay (ELISPOT) is gaining popularity for its ability to identify cellular immune responses against microbial antigens, including immunosuppressed populations such as those with HIV infecti...
متن کاملCost-efficient quantification of enzyme-linked immunospot.
Enzyme-linked immunospot (ELISPOT) analysis is a sensitive technique for the detection and quantification of antigen-specific T cells (5,7) and also for the detection of various cytokines released by immune cells (2). It is used extensively to monitor peptide-specific T cell responses in vaccination protocols (3), melanoma patients (6), and adoptive cellular immunotherapy (9) because it can det...
متن کاملImmune monitoring technology primer: the enzyme-linked immunospot (Elispot) and Fluorospot assay
Description of the technology Elispot is a workhorse for functional analysis of single immune cells. Originally established for the enumeration of specific antibody-secreting B cells in 1983 [1], the technology has been expanded to the analysis of T cells, NK cells and monocytes. The assay is typically carried out in 96 well plates with a nitrocellulose or PVDF membrane, which is coated with an...
متن کاملLocal immunodiagnosis of pulmonary tuberculosis by enzyme-linked immunospot.
Lymphocytes are crucial in the immune defence against Mycobacterium tuberculosis (MTB) infection. The aim of the present study was to ascertain whether or not MTB-specific lymphocytes are selectively compartmentalised in the lungs of patients with minimal active pulmonary tuberculosis (PTB). Patients with smear-negative MTB-culture-confirmed PTB were prospectively recruited. Differential cell c...
متن کاملAccuracy of the Bronchoalveolar Lavage Enzyme-Linked Immunospot Assay for the Diagnosis of Pulmonary Tuberculosis
Assessing of local immune response may improve the accuracy of pulmonary tuberculosis (PTB) diagnosis. Many studies have investigated diagnosing PTB based on enzyme-linked immunospot (ELISPOT) assay of bronchoalveolar lavage (BAL) fluid, but the results have been inconclusive. We meta-analyzed the available evidences on overall diagnostic performance of ELISPOT assay of BAL fluid for diagnosing...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98255st04